Biofilms exist in virtually all chronic wounds and impair wound healing, yet most commercially available antimicrobials are ineffective in managing these biofilms. A novel Antimicrobial Wound Cleanser (AWC)* and Gel (AWG)+ have been formulated with synergistic mechanisms intended to safely eliminate wound biofilms so that wound healing can progress. The aim of this study was to test the efficacy of the AWC and AWG in reducing or eliminating immature and mature biofilms.
Immature biofilm testing was performed on P. aeruginosa, S. aureus (MRSA), and C. albicans using a modification of the MBEC peg biofilm assay. Biofilms were grown on pegs within a 96 well plate, treated with AWC (10 minutes), AWG (24 hours), or commercial cleanser or gel controls, neutralized, and then placed back into growth media so that any remaining biofilms would regrow. Mature biofilm testing was performed using P. aeruginosa, S. aureus, and C. albicans grown on ex vivo pig dermal explants to evaluate the combination of AWC and AWG. Biofilms were treated for 10 minutes with AWC followed by one application of AWG, or commercial controls, and samples were taken for biofilm quantification every 24 hours for 72 hours.
In the immature biofilm model, little to no biofilm regrowth was observed after treatment with AWC or AWG, while in the mature biofilm model, the combination of AWC and AWG reduced all the biofilms by at least 6 logs by 72 hours.
The results indicate that AWC and AWG are effective against immature and mature biofilms.
* Sanara MedTech, Inc. BIAKŌSTM Antimicrobial Skin and Wound Cleanser
+ Sanara MedTech, Inc. BIAKŌSTM Antimicrobial Wound Gel