Failure of acute wounds to proceed through the norma lregulated repair process results in wound chronicization. When bacteria find the conditions to form biofilms, wound infection assumes features that differ from those caused by planktonic bacteria. Capacity to adhere to skin surface, aggregation and production of a matrix are the main differences between biofilm-associated microorganisms and their planktonic counterparts. The matrix is crucial in allowing biofilm bacteria to survive in adverse conditions such as highosmotic stress, low nutrient and oxygen availability, antibiotics and host immune responses. Diabetic foot ulcers, venous ulcers, and chronic wounds of different aetiologies have a large impact on public health, and complications such as biofilm formation contribute to sensibly increase the clinical and financial burden for their management.