Biofilms are present and contribute to chronic inflammation in most non-healing wounds, yet most commercially available antimicrobials are ineffective in managing these biofilms. An innovative Antimicrobial Wound Cleanser (AWC)* and Gel (AWG)+have been formulated with synergistic mechanisms intended to safely eliminate wound biofilms to enable wound healing progression. The aim of this study was to test the efficacy of AWC and AWG in reducing or eliminating mature biofilms in comparison to commercially available wound cleansers and gels.
Mature biofilm testing was performed using P. aeruginosa, S. aureus, and C. albicans grown on ex vivo pig dermal explants. Biofilms were treated for 10 minutes with AWC or commercial wound cleansers or 24 hours with AWG or commercial gels, and samples were taken for biofilm quantification every 24 hours for 72 hours.
In the mature biofilm model, AWC and AWG reduced or eliminated the biofilms in all microorganisms tested by 72 hours. The silver, hypochlorous-acid, sodium hypochlorite, and PHMB-based commercial controls had limited effectiveness in this biofilm model. Conclusions: The results indicate that AWC and AWG effectively reduce or eliminate mature biofilms.
*Sanara MedTech, Inc. BIAKŌS TM Antimicrobial Skin and Wound Cleanser +Sanara MedTech, Inc. BIAKŌS TM Antimicrobial Wound Gel